Hotspot

psbZ-trnG-GCC

LSC · 40402-41018 · 617 bp

Back to Carya

Pi 0.0139
MarkerSeek score 54.10
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
617
variable_sites
37
pi
0.0139
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.96
markerseek_score
54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 CTTCTCCTGATGGTTGGTCG ATTGAACCCGCATCTTCTCC 55.0 50.0 58.0 58.0 656-787 bp 1.0 87.6
psbZ-trnG-GCC_p2 TTGCTTCTCCTGATGGTTGG ATTGAACCCGCATCTTCTCC 50.0 50.0 57.8 58.0 659-790 bp 1.0 86.0
psbZ-trnG-GCC_p3 TCCTGATGGTTGGTCGAGTA ATTGAACCCGCATCTTCTCC 50.0 50.0 57.8 58.0 652-783 bp 1.0 85.9
psbZ-trnG-GCC_p4 GCTTCTCCTGATGGTTGGTC ATTGAACCCGCATCTTCTCC 55.0 50.0 58.3 58.0 657-788 bp 1.0 85.6
psbZ-trnG-GCC_p5 TTCTCCTGATGGTTGGTCGA ATTGAACCCGCATCTTCTCC 50.0 50.0 58.4 58.0 655-786 bp 1.0 84.8