Hotspot

atpH-atpI

LSC · 15227-16532 · 1306 bp

Pi 0.00481

MarkerSeek score 65.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1306
variable_sites: 23
pi: 0.00481
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1332-1446 bp	1.0	84.0
atpH-atpI_p2	GATCCCTTCTACAGCTTGGC	TTTTGCAACTTTAGCTGCGG	55.0	45.0	58.0	57.9	1417-1531 bp	1.0	83.9
atpH-atpI_p3	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGTCATC	45.0	55.0	58.0	57.8	1302-1416 bp	1.0	83.6
atpH-atpI_p4	GATCCCTTCTACAGCTTGGC	CGAATCCATGGAGGGTCATC	55.0	55.0	58.0	57.8	1387-1501 bp	1.0	83.5
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.2	57.9	1333-1447 bp	1.0	82.8