Hotspot

atpA-atpF

LSC · 13109-13182 · 74 bp

Back to Carya

Pi 0.0296
MarkerSeek score 54.70
Primer available yes
Label atpA-atpF

Feature Metadata

label_name
atpA-atpF
feature_type
igs
region
LSC
length_bp
74
variable_sites
5
pi
0.0296
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.96
markerseek_score
54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpA-atpF_p1 CCGTCGCCTACTTGAAGTAC CACGCTGTTAGAAATAACCGA 55.0 42.9 58.1 56.4 212 bp 0.1 22.3
atpA-atpF_p2 GTCGCCTACTTGAAGTACGG CACGCTGTTAGAAATAACCGA 55.0 42.9 58.1 56.4 210 bp 0.1 22.3
atpA-atpF_p3 ACTTGAAGTACGGTACCGGT CACGCTGTTAGAAATAACCGA 50.0 42.9 58.4 56.4 203 bp 0.1 20.0
atpA-atpF_p4 TCGCCTACTTGAAGTACGGT CACGCTGTTAGAAATAACCGA 50.0 42.9 58.5 56.4 209 bp 0.1 19.4
atpA-atpF_p5 CGTCGCCTACTTGAAGTACG CACGCTGTTAGAAATAACCGA 55.0 42.9 58.5 56.4 211 bp 0.1 19.3