Hotspot

matK-rps16

LSC · 3435-5084 · 1650 bp

Back to Carya

Pi 0.00453
MarkerSeek score 52.90
Primer available yes
Label matK-rps16

Feature Metadata

label_name
matK-rps16
feature_type
igs
region
LSC
length_bp
1650
variable_sites
27
pi
0.00453
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-rps16_p1 ACTCCCGAAAGATAAGTGGG TCAAAGAAGGCGGGAGTTTT 50.0 45.0 56.6 57.6 1766-1990 bp 1.0 69.3
matK-rps16_p2 ACTCCCGAAAGATAAGTGGG TTCAAAGAAGGCGGGAGTTT 50.0 45.0 56.6 57.6 1767-1991 bp 1.0 69.3
matK-rps16_p3 CTCCCGAAAGATAAGTGGGT TCAAAGAAGGCGGGAGTTTT 50.0 45.0 56.6 57.6 1765-1989 bp 1.0 69.3
matK-rps16_p4 CTCCCGAAAGATAAGTGGGT TTCAAAGAAGGCGGGAGTTT 50.0 45.0 56.6 57.6 1766-1990 bp 1.0 69.3
matK-rps16_p5 CTCCCGAAAGATAAGTGGGT TTTCAAAGAAGGCGGGAGTT 50.0 45.0 56.6 57.6 1767-1991 bp 1.0 69.3