Hotspot

psbA

LSC · 491-1552 · 1062 bp

Pi 0.508

MarkerSeek score 81.20

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 466
pi: 0.508
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	CCCCGCCGAGATTGAAAATA	AAATTCGTGTGCTTGGGAGT	50.0	45.0	58.0	58.0	1227 bp	0.5	59.5
psbA_p2	CCTTCCTTCCAGGGTCTGTA	AAATTCGTGTGCTTGGGAGT	55.0	45.0	58.0	58.0	1349 bp	0.5	59.5
psbA_p3	TCCAGGGTCTGTAATCCGTT	AAATTCGTGTGCTTGGGAGT	50.0	45.0	58.1	58.0	1342 bp	0.5	59.4
psbA_p4	TTCCAGGGTCTGTAATCCGT	AAATTCGTGTGCTTGGGAGT	50.0	45.0	58.1	58.0	1343 bp	0.5	59.4
psbA_p5	CCCCGCCGAGATTGAAAATA	TGCTTGGGAGTCCCTGATAA	50.0	50.0	58.0	58.0	1218 bp	0.5	59.4