Hotspot

rpl32-trnL-UAG

SSC · 114008-114919 · 912 bp

Back to Carum

Pi 0.0779
MarkerSeek score 79.80
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
912
variable_sites
66
pi
0.0779
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TATGGGGCAGCCTTAAAAGC AGCGTGTCTACCGATTTCAC 50.0 50.0 58.2 58.0 992-1031 bp 1.0 87.5
rpl32-trnL-UAG_p2 TATGGGGCAGCCTTAAAAGC CAGCGTGTCTACCGATTTCA 50.0 50.0 58.2 58.0 993-1032 bp 1.0 87.5
rpl32-trnL-UAG_p3 TATGGGGCAGCCTTAAAAGC CTCGCACTGCTTCCTAAGAG 50.0 55.0 58.2 58.1 1013-1052 bp 1.0 86.8
rpl32-trnL-UAG_p4 TATGGGGCAGCCTTAAAAGC CTCTCGCACTGCTTCCTAAG 50.0 55.0 58.2 58.1 1015-1054 bp 1.0 86.8
rpl32-trnL-UAG_p5 TATGGGGCAGCCTTAAAAGC CCAGAAGTGCTACTGCGAAT 50.0 50.0 58.2 58.0 1621-1662 bp 1.0 85.4