Hotspot

rpl22-rps19

LSC · 85139-85201 · 63 bp

Back to Carum

Pi 0.0484
MarkerSeek score 81.80
Primer available yes
Label rpl22-rps19

Feature Metadata

label_name
rpl22-rps19
feature_type
igs
region
LSC
length_bp
63
variable_sites
3
pi
0.0484
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22-rps19_p1 GGACCGTCCACGAATTTGAT AAATTTCCGAGGACACGCAA 50.0 45.0 58.3 58.4 223-226 bp 1.0 76.1
rpl22-rps19_p2 GGACCGTCCACGAATTTGAT AATTTCCGAGGACACGCAAA 50.0 45.0 58.3 58.4 222-225 bp 1.0 76.1
rpl22-rps19_p3 GGACCGTCCACGAATTTGAT ATTTCCGAGGACACGCAAAA 50.0 45.0 58.3 58.4 221-224 bp 1.0 76.1
rpl22-rps19_p4 GGACCGTCCACGAATTTGAT TAAATTTCCGAGGACACGCA 50.0 45.0 58.3 57.5 224-227 bp 1.0 75.6
rpl22-rps19_p5 TAGGACCGTCCACGAATTTG ATTTCCGAGGACACGCAAAA 50.0 45.0 57.6 58.4 223-226 bp 1.0 75.2