Hotspot

ycf3-trnS-GGA

LSC · 45751-46592 · 842 bp

Back to Carum

Pi 0.068
MarkerSeek score 73.40
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
842
variable_sites
45
pi
0.068
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.79
markerseek_score
73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TGTTTGTTGTCCCGACCATT TATTCGCGCCAGACCTAAAC 45.0 50.0 57.9 58.1 1579-1760 bp 1.0 86.6
ycf3-trnS-GGA_p2 CCCGATCCCAAATCATGTGT TATTCGCGCCAGACCTAAAC 50.0 50.0 57.9 58.1 1444 bp 0.5 59.0
ycf3-trnS-GGA_p3 CCCGATCCCAAATCATGTGT CCCTCGGTAAACAAAAGCCT 50.0 50.0 57.9 58.1 1097 bp 0.5 58.8
ycf3-trnS-GGA_p4 CCCGATCCCAAATCATGTGT TGGGTATGGCTCCGACTATT 50.0 50.0 57.9 57.9 1740 bp 0.5 58.8
ycf3-trnS-GGA_p5 CCCGATCCCAAATCATGTGT TTGGGTATGGCTCCGACTAT 50.0 50.0 57.9 57.9 1741 bp 0.5 58.8