Hotspot

trnY-GUA-trnE-UUC

LSC · 31603-31689 · 87 bp

Back to Carum

Pi 0.542
MarkerSeek score 77.00
Primer available yes
Label trnY-GUA-trnE-UUC

Feature Metadata

label_name
trnY-GUA-trnE-UUC
feature_type
igs
region
LSC
length_bp
87
variable_sites
45
pi
0.542
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY-GUA-trnE-UUC_p1 CCGTGTTGACAGAAAAGGGA CAATGAGCAACGATGGAGGA 50.0 50.0 58.0 58.0 522 bp 0.5 59.5
trnY-GUA-trnE-UUC_p2 TCCCGTGTTGACAGAAAAGG CAATGAGCAACGATGGAGGA 50.0 50.0 58.0 58.0 524 bp 0.5 59.5
trnY-GUA-trnE-UUC_p3 CCGTGTTGACAGAAAAGGGA AATGAGCAACGATGGAGGAC 50.0 50.0 58.0 58.0 521 bp 0.5 59.5
trnY-GUA-trnE-UUC_p4 TCCCGTGTTGACAGAAAAGG AATGAGCAACGATGGAGGAC 50.0 50.0 58.0 58.0 523 bp 0.5 59.5
trnY-GUA-trnE-UUC_p5 CTGTTCCCGTGTTGACAGAA CAATGAGCAACGATGGAGGA 50.0 50.0 58.1 58.0 528 bp 0.5 59.4