Hotspot

trnC-GCA-petN

LSC · 28569-29266 · 698 bp

Back to Carum

Pi 0.0519
MarkerSeek score 79.50
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
698
variable_sites
29
pi
0.0519
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.80
markerseek_score
79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 CGATATGGCCGAGTGGTAAG AGCCCAAGCGAGACTTACTA 55.0 50.0 57.9 58.2 657-794 bp 1.0 85.5
trnC-GCA-petN_p2 TTTCCCCAGTTCAAATCCGG AGCCCAAGCGAGACTTACTA 50.0 50.0 58.1 58.2 617-754 bp 1.0 85.5
trnC-GCA-petN_p3 CGATATGGCCGAGTGGTAAG AGAGTCCACTTCTTCCCCAT 55.0 50.0 57.9 57.6 718-855 bp 1.0 83.6
trnC-GCA-petN_p4 TTTCCCCAGTTCAAATCCGG AGAGTCCACTTCTTCCCCAT 50.0 50.0 58.1 57.6 678-815 bp 1.0 83.6
trnC-GCA-petN_p5 GGGGGACTGCAAATCCTTTT AGCCCAAGCGAGACTTACTA 50.0 50.0 58.4 58.2 635-772 bp 1.0 83.2