Hotspot

atpF-atpH

LSC · 13174-13488 · 315 bp

Back to Carum

Pi 0.0594
MarkerSeek score 82.00
Primer available yes
Label atpF-atpH

Feature Metadata

label_name
atpF-atpH
feature_type
igs
region
LSC
length_bp
315
variable_sites
17
pi
0.0594
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF-atpH_p1 ATTAAACCCGAAACTCCCGG GCCTGGTTGTAGCATTAGCA 50.0 50.0 57.9 58.2 406-431 bp 1.0 84.3
atpF-atpH_p2 CCAGTGGCCTAAAGAAACGA GCCTGGTTGTAGCATTAGCA 50.0 50.0 57.8 58.2 379-404 bp 1.0 84.0
atpF-atpH_p3 ATTAAACCCGAAACTCCCGG TGGCCTGGTTGTAGCATTAG 50.0 50.0 57.9 57.6 408-433 bp 1.0 82.9
atpF-atpH_p4 ATTAAACCCGAAACTCCCGG TGAGGCGGAGGGAAAAATAC 50.0 50.0 57.9 57.6 477-502 bp 1.0 82.5
atpF-atpH_p5 CCAGTGGCCTAAAGAAACGA TGAGGCGGAGGGAAAAATAC 50.0 50.0 57.8 57.6 450-475 bp 1.0 82.2