Hotspot

psbA-trnK-UUU

LSC · 1553-1778 · 226 bp

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Pi 0.433
MarkerSeek score 76.40
Primer available yes
Label psbA-trnK-UUU

Feature Metadata

label_name
psbA-trnK-UUU
feature_type
igs
region
LSC
length_bp
226
variable_sites
94
pi
0.433
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA-trnK-UUU_p1 CAGACGGAAACTAAGCTCCC AAGGCGGTTTGGACTGATTT 55.0 45.0 58.0 58.0 1274 bp 0.5 59.9
psbA-trnK-UUU_p2 CCAGACGGAAACTAAGCTCC AAGGCGGTTTGGACTGATTT 55.0 45.0 58.0 58.0 1275 bp 0.5 59.9
psbA-trnK-UUU_p3 CCCAGACGGAAACTAAGCTC AAGGCGGTTTGGACTGATTT 55.0 45.0 58.0 58.0 1276 bp 0.5 59.9
psbA-trnK-UUU_p4 CGGAAACTAAGCTCCCACTC AAGGCGGTTTGGACTGATTT 55.0 45.0 58.0 58.0 1270 bp 0.5 59.9
psbA-trnK-UUU_p5 AGCAGCAATGAAGGCGATAA AAGGCGGTTTGGACTGATTT 45.0 45.0 58.0 58.0 1028 bp 0.5 59.6