Hotspot

ycf1

SSC · 108400-113604 · 5205 bp

Back to Carthamus

Pi 0.00516

MarkerSeek score 31.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5205
variable_sites: 51
pi: 0.00516
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 31.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CTAGCACTGGGTAGACCTCA	TCCACGATACATCCCATCCA	55.0	50.0	57.9	57.9	1110 bp	1.0	79.2
ycf1_p2	CTAGCACTGGGTAGACCTCA	GAAAGTAACCGGCCACAGAA	55.0	50.0	57.9	58.1	436 bp	1.0	79.0
ycf1_p3	TCGTTGTGGTCGGACTCTAT	TCCACGATACATCCCATCCA	50.0	50.0	58.2	57.9	1298 bp	1.0	78.8
ycf1_p4	CTAGCACTGGGTAGACCTCA	GTGACGGTGTTCTGCCTAAA	55.0	50.0	57.9	58.1	487 bp	1.0	78.8
ycf1_p5	ATCAATTCGGTCGTTGTGGT	TCCACGATACATCCCATCCA	45.0	50.0	57.8	57.9	1308 bp	1.0	78.7