Hotspot

petB

LSC · 74548-75967 · 1420 bp

Back to Carthamus

Pi 0.00341

MarkerSeek score 51.80

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1420
variable_sites: 9
pi: 0.00341
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	TTTTGGTAGTTCGACCGTGG	ATCGTTCCTACCCCAAGTGA	50.0	50.0	58.1	58.1	1524-1525 bp	1.0	79.0
petB_p2	TTTTGGTAGTTCGACCGTGG	TCGTTCCTACCCCAAGTGAT	50.0	50.0	58.1	58.1	1523-1524 bp	1.0	79.0
petB_p3	GGTAGTTCGACCGTGGAATT	ATCGTTCCTACCCCAAGTGA	50.0	50.0	57.6	58.1	1520-1521 bp	1.0	77.1
petB_p4	GGTAGTTCGACCGTGGAATT	TCGTTCCTACCCCAAGTGAT	50.0	50.0	57.6	58.1	1519-1520 bp	1.0	77.1
petB_p5	AGAACTCCCAAGTCCTAGCT	TCGTTCCTACCCCAAGTGAT	50.0	50.0	57.4	58.1	1592-1593 bp	1.0	75.0