Hotspot

rpl32-ndhF

SSC · 124283-125328 · 1046 bp

Back to Carthamus

Pi 0.00721

MarkerSeek score 48.50

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 1046
variable_sites: 13
pi: 0.00721
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	CCGCCCAATATCCTTTCCTT	CTGGAAGTGGAAGGAAAGGT	50.0	50.0	57.6	57.0	925-1181 bp	1.0	74.3
rpl32-ndhF_p2	TGACAAAGCTTTTAACGCCG	CTGGAAGTGGAAGGAAAGGT	45.0	50.0	57.6	57.0	942-1198 bp	1.0	74.1
rpl32-ndhF_p3	TTTAACGCCGCCCAATATCC	CTGGAAGTGGAAGGAAAGGT	50.0	50.0	58.7	57.0	932-1188 bp	1.0	71.7
rpl32-ndhF_p4	GACAAAGCTTTTAACGCCGC	CTGGAAGTGGAAGGAAAGGT	50.0	50.0	58.9	57.0	941-1197 bp	1.0	69.6
rpl32-ndhF_p5	CTTTTAACGCCGCCCAATATC	CTGGAAGTGGAAGGAAAGGT	47.6	50.0	58.0	57.0	934-1190 bp	1.0	69.2