Hotspot

accD-psaI

LSC · 58290-58945 · 656 bp

Back to Carthamus

Pi 0.00483

MarkerSeek score 52.10

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 656
variable_sites: 6
pi: 0.00483
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCCAGAAGGGCTTATTCGATC	AAAGAAGCCATTGCGATTGC	47.6	45.0	57.2	58.0	837 bp	1.0	66.5
accD-psaI_p2	CCAGAAGGGCTTATTCGATCT	AAAGAAGCCATTGCGATTGC	47.6	45.0	57.2	58.0	836 bp	1.0	66.5
accD-psaI_p3	TTCCAGAAGGGCTTATTCGA	AAAGAAGCCATTGCGATTGC	45.0	45.0	56.2	58.0	838 bp	1.0	66.5
accD-psaI_p4	TCCAGAAGGGCTTATTCGATC	CCGGAAAGACTAGGCCTACT	47.6	55.0	57.2	58.2	818 bp	1.0	64.8
accD-psaI_p5	CCAGAAGGGCTTATTCGATCT	CCGGAAAGACTAGGCCTACT	47.6	55.0	57.2	58.2	817 bp	1.0	64.7