Hotspot

ndhC-trnV-UAC

LSC · 49898-51052 · 1155 bp

Back to Carthamus

Pi 0.00737

MarkerSeek score 54.80

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1155
variable_sites: 17
pi: 0.00737
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GTCCTTTGCTAATCGGAGCT	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.7	58.1	1303-1308 bp	1.0	77.8
ndhC-trnV-UAC_p2	CGGTCCTTTGCTAATCGGAG	CCGAGAAAGTCTACGGTTCG	55.0	55.0	58.4	58.1	1305-1310 bp	1.0	76.9
ndhC-trnV-UAC_p3	CTCCGGTCCTTTGCTAATCG	CCGAGAAAGTCTACGGTTCG	55.0	55.0	58.4	58.1	1308-1313 bp	1.0	76.9
ndhC-trnV-UAC_p4	GGTCCTTTGCTAATCGGAGC	CCGAGAAAGTCTACGGTTCG	55.0	55.0	58.7	58.1	1304-1309 bp	1.0	74.8
ndhC-trnV-UAC_p5	GTCCTTTGCTAATCGGAGCT	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.7	58.7	1288-1293 bp	1.0	73.2