Hotspot

trnL-UAA-trnF-GAA

LSC · 47115-47482 · 368 bp

Back to Carthamus

Pi 0.0077

MarkerSeek score 39.20

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 368
variable_sites: 5
pi: 0.0077
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 39.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AATCGTGAGGGTTCAAGTCC	TCCTCTGCTCTACCAACTGA	50.0	50.0	57.5	57.4	416-427 bp	1.0	73.5
trnL-UAA-trnF-GAA_p2	AATCGTGAGGGTTCAAGTCC	TCAGTCCTCTGCTCTACCAA	50.0	50.0	57.5	57.4	431-507 bp	1.0	73.2
trnL-UAA-trnF-GAA_p3	AATCGTGAGGGTTCAAGTCC	TTCAGTCCTCTGCTCTACCA	50.0	50.0	57.5	57.4	432-508 bp	1.0	73.2
trnL-UAA-trnF-GAA_p4	AATCGTGAGGGTTCAAGTCC	AGTCCTCTGCTCTACCAACT	50.0	50.0	57.5	57.4	429-505 bp	1.0	73.2
trnL-UAA-trnF-GAA_p5	AATCGTGAGGGTTCAAGTCC	GTCCTCTGCTCTACCAACTG	50.0	55.0	57.5	57.3	428-504 bp	1.0	72.8