Hotspot

psbC-trnS-UGA

LSC · 34445-34682 · 238 bp

Back to Carthamus

Pi 0.0151

MarkerSeek score 54.50

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 238
variable_sites: 6
pi: 0.0151
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CCTGTTCTTTCCATGACCCC	GAGAGATGGCTGAGTGGTTG	55.0	55.0	58.2	58.0	345-358 bp	1.0	81.3
psbC-trnS-UGA_p2	TCTTCGTAGGGCATTTGTGG	GAGAGATGGCTGAGTGGTTG	50.0	55.0	57.9	58.0	428-441 bp	1.0	81.2
psbC-trnS-UGA_p3	TCTTCGTAGGGCATTTGTGG	GTCTTTCGGAGAGATGGCTG	50.0	55.0	57.9	58.1	436-449 bp	1.0	80.8
psbC-trnS-UGA_p4	CCTGTTCTTTCCATGACCCC	GTCTTTCGGAGAGATGGCTG	55.0	55.0	58.2	58.1	353-366 bp	1.0	80.8
psbC-trnS-UGA_p5	TCTTCGTAGGGCATTTGTGG	TCTTTCGGAGAGATGGCTGA	50.0	50.0	57.9	58.1	435-448 bp	1.0	80.1