Hotspot

trnT-GGU-psbD

LSC · 30828-32013 · 1186 bp

Back to Carthamus

Pi 0.00608

MarkerSeek score 46.80

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1186
variable_sites: 14
pi: 0.00608
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 46.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	CGTAAGCCCTCGGTTCAAAT	GGACCAGCCTACAAAAACGA	50.0	50.0	58.3	58.1	1315-1321 bp	1.0	78.2
trnT-GGU-psbD_p2	CGTAAGCCCTCGGTTCAAAT	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.3	58.2	1305-1311 bp	1.0	77.2
trnT-GGU-psbD_p3	TAAGCCCTCGGTTCAAATCC	GGACCAGCCTACAAAAACGA	50.0	50.0	57.6	58.1	1313-1319 bp	1.0	77.1
trnT-GGU-psbD_p4	TCACTCAACCAAGCCCTTTT	GGACCAGCCTACAAAAACGA	45.0	50.0	57.5	58.1	1366-1372 bp	1.0	76.7
trnT-GGU-psbD_p5	TTCACTCAACCAAGCCCTTT	GGACCAGCCTACAAAAACGA	45.0	50.0	57.5	58.1	1367-1373 bp	1.0	76.7