Hotspot

trnG-UCC-trnT-GGU

LSC · 30643-30755 · 113 bp

Back to Carthamus

Pi 0.0354
MarkerSeek score 63.20
Primer available yes
Label trnG-UCC-trnT-GGU

Feature Metadata

label_name
trnG-UCC-trnT-GGU
feature_type
igs
region
LSC
length_bp
113
variable_sites
8
pi
0.0354
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnT-GGU_p1 TGAAAACCGAAAGACCCCTT ATTTGAACCGAGGGCTTACG 45.0 50.0 57.3 58.3 248 bp 0.25 41.9
trnG-UCC-trnT-GGU_p2 TGAAAACCGAAAGACCCCTT GGATTTGAACCGAGGGCTTA 45.0 50.0 57.3 57.6 250 bp 0.25 40.8
trnG-UCC-trnT-GGU_p3 ATGAAAACCGAAAGACCCCT ATTTGAACCGAGGGCTTACG 45.0 50.0 57.0 58.3 249 bp 0.25 40.1
trnG-UCC-trnT-GGU_p4 ATGAAAACCGAAAGACCCCT GGATTTGAACCGAGGGCTTA 45.0 50.0 57.0 57.6 251 bp 0.25 38.9
trnG-UCC-trnT-GGU_p5 ATGAAAACCGAAAGACCCCTT ATTTGAACCGAGGGCTTACG 42.9 50.0 57.8 58.3 249 bp 0.25 38.0