Hotspot

ndhA

SSC · 124454-126744 · 2291 bp

Back to Carpinus

Pi 0.00116

MarkerSeek score 56.10

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2291
variable_sites: 13
pi: 0.00116
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGACCCCAGTTACCATAGGG	CCCGCCGGGTTTTATCAATT	55.0	50.0	58.1	58.9	2509-2528 bp	1.0	72.9
ndhA_p2	TGACCCCAGTTACCATAGGG	CCGCCGGGTTTTATCAATTT	55.0	45.0	58.1	57.0	2508-2527 bp	1.0	72.4
ndhA_p3	GACCCCAGTTACCATAGGGA	CCCGCCGGGTTTTATCAATT	55.0	50.0	57.8	58.9	2508-2527 bp	1.0	72.3
ndhA_p4	GACCCCAGTTACCATAGGGA	CCGCCGGGTTTTATCAATTT	55.0	45.0	57.8	57.0	2507-2526 bp	1.0	71.9
ndhA_p5	TGACCCCAGTTACCATAGGG	GCCGGGTTTTATCAATTTGCA	55.0	42.9	58.1	58.0	2506-2525 bp	1.0	71.8