Hotspot

rpl32-trnL-TAG

SSC · 117735-118752 · 1018 bp

Back to Carpinus

Pi 0.00182
MarkerSeek score 58.40
Primer available yes
Label rpl32-trnL-TAG

Feature Metadata

label_name
rpl32-trnL-TAG
feature_type
igs
region
SSC
length_bp
1018
variable_sites
8
pi
0.00182
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.98
markerseek_score
58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-TAG_p1 GGAGGGGTATTGGGTAGCAT AGCGTGTCTACCGATTTCAC 55.0 50.0 58.6 58.0 1112-1157 bp 1.0 79.1
rpl32-trnL-TAG_p2 ATGGAGGGGTATTGGGTAGC AGCGTGTCTACCGATTTCAC 55.0 50.0 58.6 58.0 1114-1159 bp 1.0 79.0
rpl32-trnL-TAG_p3 ATGGAGGGGTATTGGGTAGC CAGCGTGTCTACCGATTTCA 55.0 50.0 58.6 58.0 1115-1160 bp 1.0 79.0
rpl32-trnL-TAG_p4 GGAGGGGTATTGGGTAGCAT CAGCGTGTCTACCGATTTCA 55.0 50.0 58.6 58.0 1113-1158 bp 1.0 79.0
rpl32-trnL-TAG_p5 GGAGGGGTATTGGGTAGCAT TCCTAAGAGCAGCGTGTCTA 55.0 50.0 58.6 57.9 1122-1167 bp 1.0 78.1