Hotspot

psbE-petL

LSC · 68740-69792 · 1053 bp

Back to Carpinus

Pi 0.00233

MarkerSeek score 56.30

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1053
variable_sites: 9
pi: 0.00233
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	AACCGCTAGTAGAAAACCGA	50.0	45.0	57.8	56.6	1122-1143 bp	1.0	68.2
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	ACCGCTAGTAGAAAACCGAA	50.0	45.0	57.8	56.6	1121-1142 bp	1.0	68.2
psbE-petL_p3	ACGAATAACCAACCCGCAAT	AACCGCTAGTAGAAAACCGA	45.0	45.0	58.2	56.6	1208-1229 bp	1.0	68.1
psbE-petL_p4	ACGAATAACCAACCCGCAAT	ACCGCTAGTAGAAAACCGAA	45.0	45.0	58.2	56.6	1207-1228 bp	1.0	68.1
psbE-petL_p5	TGCTGACGAATAACCAACCC	ACCGCTAGTAGAAAACCGAA	50.0	45.0	58.2	56.6	1212-1233 bp	1.0	68.0