Hotspot

ndhC-trnM-CAT

LSC · 54507-55926 · 1420 bp

Back to Carpinus

Pi 0.00336

MarkerSeek score 60.30

Primer available yes

Label ndhC-trnM-CAT

Feature Metadata

label_name: ndhC-trnM-CAT
feature_type: igs
region: LSC
length_bp: 1420
variable_sites: 16
pi: 0.00336
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnM-CAT_p1	GGGGCTAAAACTCCCGAAAT	CCCGCCGTATGAAAGCAATA	50.0	50.0	57.9	58.4	1559-1611 bp	1.0	77.5
ndhC-trnM-CAT_p2	CCTTTGCTAATCGGGGCTAA	CCCGCCGTATGAAAGCAATA	50.0	50.0	57.7	58.4	1571-1623 bp	1.0	75.8
ndhC-trnM-CAT_p3	GGGGCTAAAACTCCCGAAAT	CCGCCGTATGAAAGCAATAC	50.0	50.0	57.9	57.3	1558-1610 bp	1.0	75.5
ndhC-trnM-CAT_p4	GGGGCTAAAACTCCCGAAAT	TTTGAACCAATGACTCCCGC	50.0	50.0	57.9	58.8	1574-1626 bp	1.0	74.7
ndhC-trnM-CAT_p5	CTTCTCTGGCCCTTTGCTAA	CCCGCCGTATGAAAGCAATA	50.0	50.0	57.5	58.4	1581-1633 bp	1.0	74.6