Hotspot

trnT-TGT-trnF-GAA

LSC · 50131-51920 · 1790 bp

Back to Carpinus

Pi 0.00302

MarkerSeek score 57.20

Primer available yes

Label trnT-TGT-trnF-GAA

Feature Metadata

label_name: trnT-TGT-trnF-GAA
feature_type: igs
region: LSC
length_bp: 1790
variable_sites: 15
pi: 0.00302
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-TGT-trnF-GAA_p1	GCGATGCTCTAACCTCTGAG	TCTACCAGCTGAGCTATCCC	55.0	55.0	57.9	58.0	1804-1843 bp	1.0	82.4
trnT-TGT-trnF-GAA_p2	AAAGCCTGCTATCGGAATCG	TCTACCAGCTGAGCTATCCC	50.0	55.0	58.1	58.0	1849-1888 bp	1.0	82.3
trnT-TGT-trnF-GAA_p3	AAAGCCTGCTATCGGAATCG	TTTGAACTGGTGACACGAGG	50.0	50.0	58.1	58.1	1885-1924 bp	1.0	81.9
trnT-TGT-trnF-GAA_p4	GCGATGCTCTAACCTCTGAG	TTTGAACTGGTGACACGAGG	55.0	50.0	57.9	58.1	1840-1879 bp	1.0	81.9
trnT-TGT-trnF-GAA_p5	AAAGCCTGCTATCGGAATCG	GTGCCAGGAACCAGATTTGA	50.0	50.0	58.1	58.1	1900-1939 bp	1.0	81.5