Hotspot

trnT-GGT-psbD

LSC · 34899-36326 · 1428 bp

Back to Carpinus

Pi 0.087
MarkerSeek score 89.90
Primer available yes
Label trnT-GGT-psbD

Feature Metadata

label_name
trnT-GGT-psbD
feature_type
igs
region
LSC
length_bp
1428
variable_sites
303
pi
0.087
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
89.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGT-psbD_p1 GTGGTAGAGTAACGCCATGG ACAAAAACGAAACGGTCCCT 55.0 45.0 58.1 58.2 1294-1606 bp 1.0 89.5
trnT-GGT-psbD_p2 GTGGTAGAGTAACGCCATGG GACCGGACCAACCTACAAAA 55.0 50.0 58.1 57.7 1308-1620 bp 1.0 89.4
trnT-GGT-psbD_p3 TCAGTGGTAGAGTAACGCCA ACAAAAACGAAACGGTCCCT 50.0 45.0 58.1 58.2 1297-1609 bp 1.0 89.3
trnT-GGT-psbD_p4 TCAGTGGTAGAGTAACGCCA GACCGGACCAACCTACAAAA 50.0 50.0 58.1 57.7 1311-1623 bp 1.0 89.2
trnT-GGT-psbD_p5 GTGGTAGAGTAACGCCATGG TAGACCGGACCAACCTACAA 55.0 50.0 58.1 57.7 1310-1622 bp 1.0 89.0