Hotspot

trnS-GCT-trnR-TCT

LSC · 9365-11415 · 2051 bp

Back to Carpinus

Pi 0.00977
MarkerSeek score 70.30
Primer available yes
Label trnS-GCT-trnR-TCT

Feature Metadata

label_name
trnS-GCT-trnR-TCT
feature_type
igs
region
LSC
length_bp
2051
variable_sites
54
pi
0.00977
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.97
markerseek_score
70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCT-trnR-TCT_p1 CAATCCGACGCTTTAGTCCA AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 2027-2139 bp 1.0 69.7
trnS-GCT-trnR-TCT_p2 AATCCGACGCTTTAGTCCAC AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 2026-2138 bp 1.0 69.6
trnS-GCT-trnR-TCT_p3 CAATCCGACGCTTTAGTCCA AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.0 57.9 2033-2145 bp 1.0 69.0
trnS-GCT-trnR-TCT_p4 AATCCGACGCTTTAGTCCAC AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.0 57.9 2032-2144 bp 1.0 69.0
trnS-GCT-trnR-TCT_p5 ACAAAAGCGGAAAGAGAGGG AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.1 56.9 2087-2199 bp 1.0 68.8