Hotspot

trnN-GUU-trnR-ACG

IRa · 128203-128579 · 377 bp

Back to Caroxylon

Pi 0.0283

MarkerSeek score 65.90

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 377
variable_sites: 16
pi: 0.0283
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	TGGGTTGAGAACGGGAATTG	CAGTGGATTAGAGCACGTGG	50.0	55.0	58.1	58.4	555-559 bp	1.0	79.3
trnN-GUU-trnR-ACG_p2	TGGTCGTAGGTTCGAATCCT	CAGTGGATTAGAGCACGTGG	50.0	55.0	58.2	58.4	465-469 bp	1.0	79.3
trnN-GUU-trnR-ACG_p3	GGGTTGAGAACGGGAATTGA	CAGTGGATTAGAGCACGTGG	50.0	55.0	57.8	58.4	554-558 bp	1.0	78.5
trnN-GUU-trnR-ACG_p4	CGATTGGTCGTAGGTTCGAA	CAGTGGATTAGAGCACGTGG	50.0	55.0	57.7	58.4	469-473 bp	1.0	78.4
trnN-GUU-trnR-ACG_p5	TTCCTCAGTAGCTCAGTGGT	CAGTGGATTAGAGCACGTGG	50.0	55.0	57.7	58.4	508-512 bp	1.0	77.8