Hotspot

rps15-ycf1

SSC · 121889-122185 · 297 bp

Back to Caroxylon

Pi 0.0393

MarkerSeek score 68.30

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 297
variable_sites: 17
pi: 0.0393
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TGCATTGATGGATGGTGAGG	TCGGGTAAAAACCTGCAGTC	50.0	50.0	57.9	58.1	930-968 bp	1.0	84.3
rps15-ycf1_p2	GATGGTGAGGGCCCATATTG	TCGGGTAAAAACCTGCAGTC	55.0	50.0	58.1	58.1	920-958 bp	1.0	84.2
rps15-ycf1_p3	GAGAACGCCATGCATTGATG	TCGGGTAAAAACCTGCAGTC	50.0	50.0	57.9	58.1	940-978 bp	1.0	83.8
rps15-ycf1_p4	ATTGTTCGGGCCCATTTACA	TCGGGTAAAAACCTGCAGTC	45.0	50.0	57.8	58.1	1127-1165 bp	1.0	82.1
rps15-ycf1_p5	TTTCCTAATTGTTCGGGCCC	TCGGGTAAAAACCTGCAGTC	50.0	50.0	58.2	58.1	1172 bp	0.666667	64.7