Hotspot

trnN-GUU-ndhF

IRb · 106638-108280 · 1643 bp

Back to Caroxylon

Pi 0.0165

MarkerSeek score 57.00

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 1643
variable_sites: 39
pi: 0.0165
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	TGGGTCTTTTGTCGAAGTGG	ATTCCGAATGGCTTTGGTGT	50.0	45.0	58.0	58.1	2116-2139 bp	1.0	82.4
trnN-GUU-ndhF_p2	GGCAGAATAGCAGAGCAAGT	ATTCCGAATGGCTTTGGTGT	50.0	45.0	58.0	58.1	2009-2028 bp	1.0	82.2
trnN-GUU-ndhF_p3	GCTTCCCTACTCTTAACGGC	ATTCCGAATGGCTTTGGTGT	55.0	45.0	58.1	58.1	1906-1925 bp	1.0	82.1
trnN-GUU-ndhF_p4	GAACTTGGGTGTGGGTCTTT	ATTCCGAATGGCTTTGGTGT	50.0	45.0	57.9	58.1	2127-2150 bp	1.0	82.1
trnN-GUU-ndhF_p5	CAAAGGGGAAAGGTCCTTCC	ATTCCGAATGGCTTTGGTGT	55.0	45.0	58.1	58.1	2219-2223 bp	0.666667	65.9