Hotspot

trnR-ACG-trnN-GUU

IRb · 106189-106565 · 377 bp

Back to Caroxylon

Pi 0.0283

MarkerSeek score 65.90

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 377
variable_sites: 16
pi: 0.0283
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGTGGATTAGAGCACGTGG	TGGGTTGAGAACGGGAATTG	55.0	50.0	58.4	58.1	555-559 bp	1.0	79.3
trnR-ACG-trnN-GUU_p2	CAGTGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	58.4	58.2	465-469 bp	1.0	79.3
trnR-ACG-trnN-GUU_p3	CAGTGGATTAGAGCACGTGG	GGGTTGAGAACGGGAATTGA	55.0	50.0	58.4	57.8	554-558 bp	1.0	78.5
trnR-ACG-trnN-GUU_p4	CAGTGGATTAGAGCACGTGG	CGATTGGTCGTAGGTTCGAA	55.0	50.0	58.4	57.7	469-473 bp	1.0	78.4
trnR-ACG-trnN-GUU_p5	CAGTGGATTAGAGCACGTGG	TTCCTCAGTAGCTCAGTGGT	55.0	50.0	58.4	57.7	508-512 bp	1.0	77.8