Hotspot

trnP-UGG-psaJ

LSC · 66277-66694 · 418 bp

Back to Caroxylon

Pi 0.0099

MarkerSeek score 65.40

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 418
variable_sites: 6
pi: 0.0099
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	TGTCAACGCATCTGGGAAAA	50.0	45.0	58.1	58.0	567-587 bp	1.0	82.6
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	TGTCAACGCATCTGGGAAAA	50.0	45.0	58.1	58.0	566-586 bp	1.0	82.6
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	TGTCAACGCATCTGGGAAAA	50.0	45.0	58.1	58.0	578-598 bp	1.0	82.5
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	TGTCAACGCATCTGGGAAAA	50.0	45.0	58.1	58.0	577-597 bp	1.0	82.5
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	TGTCAACGCATCTGGGAAAA	50.0	45.0	58.1	58.0	580-600 bp	1.0	82.5