Hotspot

rbcL-accD

LSC · 56665-57350 · 686 bp

Back to Caroxylon

Pi 0.0267

MarkerSeek score 65.90

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 686
variable_sites: 24
pi: 0.0267
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	TGCCTGCTTTAACCGAGATC	CTCCCAATGCGGATTTGAGA	50.0	50.0	58.0	58.0	1322-1497 bp	1.0	85.4
rbcL-accD_p2	AATGAGGGACGTGATCTTGC	CTCCCAATGCGGATTTGAGA	50.0	50.0	58.0	58.0	1188-1363 bp	1.0	85.4
rbcL-accD_p3	TGCAATGCACGCAGTTATTG	CTCCCAATGCGGATTTGAGA	45.0	50.0	58.0	58.0	1597-1772 bp	1.0	85.2
rbcL-accD_p4	TTGAATGCTACTGCGGGTAC	CTCCCAATGCGGATTTGAGA	50.0	50.0	58.0	58.0	1764-1939 bp	1.0	85.1
rbcL-accD_p5	GCGGGTACATGTGAAGACAT	CTCCCAATGCGGATTTGAGA	50.0	50.0	58.0	58.0	1752-1927 bp	1.0	85.0