Hotspot

ndhC-trnV-UAC

LSC · 50629-51414 · 786 bp

Back to Caroxylon

Pi 0.0187

MarkerSeek score 72.10

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 786
variable_sites: 22
pi: 0.0187
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCCTTTGCTACTCGGAGCTA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	922-931 bp	1.0	80.8
ndhC-trnV-UAC_p2	TTCTCGGGTCCTTTGCTACT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.4	57.8	930-939 bp	1.0	79.0
ndhC-trnV-UAC_p3	TTTCTCGGGTCCTTTGCTAC	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.5	57.8	931-940 bp	1.0	78.1
ndhC-trnV-UAC_p4	TCCTTTGCTACTCGGAGCTA	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	921-930 bp	1.0	78.0
ndhC-trnV-UAC_p5	TCCTTTGCTACTCGGAGCTA	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.9	58.7	910-919 bp	1.0	76.9