Hotspot

trnS-GGA-rps4

LSC · 45229-45567 · 339 bp

Back to Caroxylon

Pi 0.0295

MarkerSeek score 68.30

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: LSC
length_bp: 339
variable_sites: 15
pi: 0.0295
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	AGGCTTTTGTTTACCGAGGG	TGACTCTTCACCCATTCCCA	50.0	50.0	58.1	58.3	495-496 bp	1.0	79.8
trnS-GGA-rps4_p2	GGCTTTTGTTTACCGAGGGT	TGACTCTTCACCCATTCCCA	50.0	50.0	58.4	58.3	494-495 bp	1.0	77.4
trnS-GGA-rps4_p3	AGGCTTTTGTTTACCGAGGG	TTGACTCTTCACCCATTCCC	50.0	50.0	58.1	57.1	496-497 bp	1.0	74.8
trnS-GGA-rps4_p4	AGGCTTTTGTTTACCGAGGG	GACTCTTCACCCATTCCCAT	50.0	50.0	58.1	56.9	494-495 bp	1.0	73.0
trnS-GGA-rps4_p5	TTCGAATCCCTCTCTTTCCG	TGACTCTTCACCCATTCCCA	50.0	50.0	57.0	58.3	475-476 bp	1.0	72.9