Hotspot

psaA-ycf3

LSC · 41741-42470 · 730 bp

Back to Caroxylon

Pi 0.0192

MarkerSeek score 69.90

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 730
variable_sites: 21
pi: 0.0192
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGAAAAATGACCGGGTCTGG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.1	58.0	890-927 bp	1.0	84.2
psaA-ycf3_p2	TGAAAAATGACCGGGTCTGG	ATTGGTTGAAGATCACGGGG	50.0	50.0	58.1	57.9	824-861 bp	1.0	84.0
psaA-ycf3_p3	TGAAAAATGACCGGGTCTGG	CGATCAAGCCGCTGAGTATT	50.0	50.0	58.1	58.1	892-929 bp	1.0	83.4
psaA-ycf3_p4	TGAAAAATGACCGGGTCTGG	AGCCGCTGAGTATTGGAAAC	50.0	50.0	58.1	58.3	886-923 bp	1.0	82.5
psaA-ycf3_p5	TGAAAAATGACCGGGTCTGG	CAAGCCGCTGAGTATTGGAA	50.0	50.0	58.1	58.3	888-925 bp	1.0	82.5