Hotspot

petN-psbM

LSC · 28247-28948 · 702 bp

Back to Caroxylon

Pi 0.0247

MarkerSeek score 71.30

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 702
variable_sites: 26
pi: 0.0247
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ATTGTAAGTCTCGCTTGGGC	AAAACGGTCAGCCAAAATGA	50.0	40.0	58.3	56.4	807-810 bp	1.0	68.6
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	AAAACGGTCAGCCAAAATGA	50.0	40.0	57.6	56.4	745-748 bp	1.0	68.2
petN-psbM_p3	ATTGTAAGTCTCGCTTGGGC	AAACGGTCAGCCAAAATGAT	50.0	40.0	58.3	56.2	806-809 bp	1.0	66.8
petN-psbM_p4	ATTGTAAGTCTCGCTTGGGC	AACGGTCAGCCAAAATGATT	50.0	40.0	58.3	56.2	805-808 bp	1.0	66.8
petN-psbM_p5	ATTGTAAGTCTCGCTTGGGC	AAAAACGGTCAGCCAAAATGA	50.0	38.1	58.3	57.2	808-811 bp	1.0	66.6