Hotspot

atpH-atpI

LSC · 13498-14199 · 702 bp

Back to Caroxylon

Pi 0.0199

MarkerSeek score 67.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 702
variable_sites: 21
pi: 0.0199
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACGGAAGCAGCAGAAATCA	TTTTGCAACTTTAGCCGCAG	45.0	45.0	58.1	57.9	787-792 bp	1.0	80.8
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	TTTTGCAACTTTAGCCGCAG	50.0	45.0	57.9	57.9	845-850 bp	1.0	80.6
atpH-atpI_p3	AACGGAAGCAGCAGAAATCA	CGAATCCATGGAGGGTCATC	45.0	55.0	58.1	57.8	757-762 bp	1.0	80.6
atpH-atpI_p4	AGCCAATCCAGCAGCAATAA	TTTTGCAACTTTAGCCGCAG	45.0	45.0	57.9	57.9	805-810 bp	1.0	80.4
atpH-atpI_p5	TACCTTGACCAACTCCAGGT	CGAATCCATGGAGGGTCATC	50.0	55.0	57.9	57.8	815-820 bp	1.0	80.3