Hotspot

petA-psbJ

LSC · 63218-64007 · 790 bp

Back to Carlina

Pi 0.011
MarkerSeek score 53.10
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
790
variable_sites
16
pi
0.011
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 CTGACGGGAGCAAGAGTAAC AGCCCATTCGGAAGGATTTC 55.0 50.0 58.0 57.9 1371-1383 bp 1.0 81.5
petA-psbJ_p2 CTGACGGGAGCAAGAGTAAC TGGAGGGAAGTGGGGTAAAT 55.0 50.0 58.0 57.9 1296-1308 bp 1.0 81.5
petA-psbJ_p3 CTGACGGGAGCAAGAGTAAC TTAGGAGGCCTCAATGACCA 55.0 50.0 58.0 58.0 1698-1710 bp 1.0 81.3
petA-psbJ_p4 CTGACGGGAGCAAGAGTAAC TCCCTATTCATTGCGGGTTG 55.0 50.0 58.0 57.9 1862-1874 bp 1.0 81.0
petA-psbJ_p5 GAGCTATCGCCCCAATCAAA TTAGGAGGCCTCAATGACCA 50.0 50.0 58.0 58.0 1871-1883 bp 1.0 81.0