Hotspot

ycf4-cemA

LSC · 60541-61324 · 784 bp

Back to Carlina

Pi 0.00744

MarkerSeek score 63.90

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 784
variable_sites: 11
pi: 0.00744
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GAGCCATTCCATTGACTCGT	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.0	58.1	964-1022 bp	1.0	80.0
ycf4-cemA_p2	TTGGCCTATTTCTTGCGTGT	GAGAAATCCACCAGGGCAAA	45.0	50.0	58.1	58.1	896-954 bp	1.0	79.6
ycf4-cemA_p3	CTCCACGGGAAATGGAACAA	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.1	58.1	928-986 bp	1.0	79.5
ycf4-cemA_p4	GAGCCATTCCATTGACTCGT	AAATCCACCAGGGCAAAACA	50.0	45.0	58.0	58.2	961-1019 bp	1.0	79.0
ycf4-cemA_p5	GAGCCATTCCATTGACTCGT	AATCCACCAGGGCAAAACAA	50.0	45.0	58.0	58.2	960-1018 bp	1.0	79.0