Hotspot

trnT-GGU-psbD

LSC · 31474-32679 · 1206 bp

Back to Carlina

Pi 0.00931

MarkerSeek score 53.90

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1206
variable_sites: 18
pi: 0.00931
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAATGCCACGG	TTTACCAAGGGCTATAGTCA	55.0	40.0	58.1	53.0	1283-1302 bp	1.0	42.1
trnT-GGU-psbD_p2	CGTAAGCCCTCGGTTCAAAT	TTTACCAAGGGCTATAGTCA	50.0	40.0	58.3	53.0	1258-1277 bp	1.0	42.1
trnT-GGU-psbD_p3	GTGACCCGTTCAGGAATTCA	TTTACCAAGGGCTATAGTCA	50.0	40.0	57.8	53.0	1326-1345 bp	1.0	42.0
trnT-GGU-psbD_p4	CAGTGACCCGTTCAGGAATT	TTTACCAAGGGCTATAGTCA	50.0	40.0	57.8	53.0	1328-1347 bp	1.0	42.0
trnT-GGU-psbD_p5	TACAGTGACCCGTTCAGGAA	TTTACCAAGGGCTATAGTCA	50.0	40.0	58.3	53.0	1330-1349 bp	1.0	42.0