Hotspot

atpA-trnR-UCU

LSC · 29984-30171 · 188 bp

Back to Carlina

Pi 0.0363

MarkerSeek score 74.00

Primer available yes

Label atpA-trnR-UCU

Feature Metadata

label_name: atpA-trnR-UCU
feature_type: igs
region: LSC
length_bp: 188
variable_sites: 12
pi: 0.0363
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-trnR-UCU_p1	CATTCACCGAGGAAGCAGAA	GCAATGAAAAGCGTCCATTGT	50.0	42.9	57.9	58.3	356-389 bp	1.0	73.7
atpA-trnR-UCU_p2	AAGACATTCACCGAGGAAGC	GCAATGAAAAGCGTCCATTGT	50.0	42.9	57.9	58.3	360-393 bp	1.0	73.7
atpA-trnR-UCU_p3	ATTCAGGAACAGGTGGAACG	GCAATGAAAAGCGTCCATTGT	50.0	42.9	57.8	58.3	318-351 bp	1.0	73.6
atpA-trnR-UCU_p4	CATTCACCGAGGAAGCAGAA	GCAATGAAAAGCGTCCATTG	50.0	45.0	57.9	56.6	356-389 bp	1.0	72.9
atpA-trnR-UCU_p5	AAGACATTCACCGAGGAAGC	GCAATGAAAAGCGTCCATTG	50.0	45.0	57.9	56.6	360-393 bp	1.0	72.9