Hotspot

trnH-GUG-psbA

LSC · 118-492 · 375 bp

Back to Carlina

Pi 0.00578

MarkerSeek score 44.30

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 375
variable_sites: 4
pi: 0.00578
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	GAGCTTAGTTTCCGTCTGGG	55.0	55.0	58.1	58.0	1065-1091 bp	1.0	79.4
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	GAGTGGGAGCTTAGTTTCCG	55.0	55.0	58.1	58.0	1071-1097 bp	1.0	79.4
trnH-GUG-psbA_p3	GATCCACTTGGCTACATCCG	GGAGCTTAGTTTCCGTCTGG	55.0	55.0	58.1	58.0	1066-1092 bp	1.0	79.4
trnH-GUG-psbA_p4	GATCCACTTGGCTACATCCG	GGGAGCTTAGTTTCCGTCTG	55.0	55.0	58.1	58.0	1067-1093 bp	1.0	79.4
trnH-GUG-psbA_p5	CAATCCACTGCCTTGATCCA	GAGCTTAGTTTCCGTCTGGG	50.0	55.0	57.9	58.0	1079-1105 bp	1.0	79.4