Hotspot

ycf3

LSC · 43703-45724 · 2022 bp

Back to Carissa

Pi 0.00148

MarkerSeek score 54.50

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 2022
variable_sites: 3
pi: 0.00148
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	TGACTGATGGGGTCAACAAA	TGGGATCATTTTCTCACGAGAG	45.0	45.5	57.0	57.9	2174-2175 bp	1.0	54.8
ycf3_p2	TTGACTGATGGGGTCAACAA	TGGGATCATTTTCTCACGAGAG	45.0	45.5	57.0	57.9	2175-2176 bp	1.0	54.8
ycf3_p3	TGACTGATGGGGTCAACAAA	GGATCATTTTCTCACGAGAGGT	45.0	45.5	57.0	57.9	2172-2173 bp	1.0	54.8
ycf3_p4	TTGACTGATGGGGTCAACAA	GGATCATTTTCTCACGAGAGGT	45.0	45.5	57.0	57.9	2173-2174 bp	1.0	54.8
ycf3_p5	TGACTGATGGGGTCAACAAA	GTGGGATCATTTTCTCACGAGA	45.0	45.5	57.0	58.1	2175-2176 bp	1.0	54.7