Hotspot

rps16

LSC · 5132-6243 · 1112 bp

Back to Carissa

Pi 0.0018
MarkerSeek score 59.80
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
1112
variable_sites
2
pi
0.0018
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 TACGAATAGAGCGGGAAAGG ACGATGTCTACGAACCAATGA 50.0 42.9 56.8 57.1 1290-1292 bp 1.0 55.9
rps16_p2 CGAATAGAGCGGGAAAGGG ACGATGTCTACGAACCAATGA 57.9 42.9 57.7 57.1 1288-1290 bp 1.0 54.9
rps16_p3 TACGAATAGAGCGGGAAAGG CGATGTCTACGAACCAATGAC 50.0 47.6 56.8 56.8 1289-1291 bp 1.0 53.4
rps16_p4 ACGAATAGAGCGGGAAAGGG ACGATGTCTACGAACCAATGA 55.0 42.9 59.5 57.1 1289-1291 bp 1.0 52.9
rps16_p5 TACGAATAGAGCGGGAAAGG CGATGTCTACGAACCAATGACT 50.0 45.5 56.8 58.2 1289-1291 bp 1.0 52.9