Hotspot

psbE

LSC · 65986-66237 · 252 bp

Back to Carissa

Pi 0.00397

MarkerSeek score 45.90

Primer available yes

Label psbE

Feature Metadata

label_name: psbE
feature_type: gene
region: LSC
length_bp: 252
variable_sites: 1
pi: 0.00397
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE_p1	GAACAGCCAACCATCGTACT	TGGGTTTCGGTGAATTGTCA	50.0	45.0	57.9	57.6	391 bp	1.0	76.6
psbE_p2	AACAGCCAACCATCGTACTG	TGGGTTTCGGTGAATTGTCA	50.0	45.0	58.2	57.6	390 bp	1.0	75.9
psbE_p3	GTGAACAGCCAACCATCGTA	TGGGTTTCGGTGAATTGTCA	50.0	45.0	58.2	57.6	393 bp	1.0	75.8
psbE_p4	TGAACAGCCAACCATCGTAC	TGGGTTTCGGTGAATTGTCA	50.0	45.0	58.2	57.6	392 bp	1.0	75.8
psbE_p5	AGACGGTAGGTACAGCTAGG	TGGGTTTCGGTGAATTGTCA	55.0	45.0	57.7	57.6	415 bp	1.0	74.6