Hotspot

ndhK

LSC · 50694-51377 · 684 bp

Back to Carissa

Pi 0.00146

MarkerSeek score 59.60

Primer available yes

Label ndhK

Feature Metadata

label_name: ndhK
feature_type: gene
region: LSC
length_bp: 684
variable_sites: 1
pi: 0.00146
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK_p1	ACAAACGACCCTGCATCTTT	GTTTATGCATGGCGAAAGGG	45.0	50.0	58.0	57.8	883 bp	1.0	78.3
ndhK_p2	ACAAACGACCCTGCATCTTT	GGGGCATTGGAATGGTCTTA	45.0	50.0	58.0	57.5	865 bp	1.0	76.3
ndhK_p3	GACAAACGACCCTGCATCTT	GTTTATGCATGGCGAAAGGG	50.0	50.0	58.5	57.8	884 bp	1.0	74.6
ndhK_p4	ACAAACGACCCTGCATCTTT	TTTATGCATGGCGAAAGGGG	45.0	50.0	58.0	58.9	882 bp	1.0	72.8
ndhK_p5	GACAAACGACCCTGCATCTT	GGGGCATTGGAATGGTCTTA	50.0	50.0	58.5	57.5	866 bp	1.0	72.7