Hotspot

trnL-CAA-ycf2

IRa · 145524-146225 · 702 bp

Back to Carissa

Pi 0.0057
MarkerSeek score 62.00
Primer available yes
Label trnL-CAA-ycf2

Feature Metadata

label_name
trnL-CAA-ycf2
feature_type
igs
region
IRa
length_bp
702
variable_sites
4
pi
0.0057
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-CAA-ycf2_p1 AAATGGTAGACACGCGAGAC ATGGCTTTTCCCGGATGAAA 50.0 45.0 58.0 57.8 811 bp 1.0 78.5
trnL-CAA-ycf2_p2 AAATGGTAGACACGCGAGAC TGGCTTTTCCCGGATGAAAT 50.0 45.0 58.0 57.8 810 bp 1.0 78.5
trnL-CAA-ycf2_p3 TGAAATGGTAGACACGCGAG ATGGCTTTTCCCGGATGAAA 50.0 45.0 58.0 57.8 813 bp 1.0 78.5
trnL-CAA-ycf2_p4 TGAAATGGTAGACACGCGAG TGGCTTTTCCCGGATGAAAT 50.0 45.0 58.0 57.8 812 bp 1.0 78.5
trnL-CAA-ycf2_p5 AAATGGTAGACACGCGAGAC AAGATGGCTTTTCCCGGATG 50.0 50.0 58.0 58.2 814 bp 1.0 78.5