Hotspot

ndhB-trnL-CAA

IRa · 144892-145442 · 551 bp

Back to Carissa

Pi 0
MarkerSeek score 34.60
Primer available yes
Label ndhB-trnL-CAA

Feature Metadata

label_name
ndhB-trnL-CAA
feature_type
igs
region
IRa
length_bp
551
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
34.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhB-trnL-CAA_p1 TGCAATTGCTCAGGATACACT GTCTCGCGTGTCTACCATTT 42.9 50.0 57.6 58.0 611 bp 1.0 69.1
ndhB-trnL-CAA_p2 TGCAATTGCTCAGGATACACT CTCGCGTGTCTACCATTTCA 42.9 50.0 57.6 58.0 609 bp 1.0 69.1
ndhB-trnL-CAA_p3 TGCAATTGCTCAGGATACACT TACCATTTCACCACCAAGGC 42.9 50.0 57.6 58.1 599 bp 1.0 68.5
ndhB-trnL-CAA_p4 TGCAATTGCTCAGGATACACT GCGTGTCTACCATTTCACCA 42.9 50.0 57.6 58.2 606 bp 1.0 67.6
ndhB-trnL-CAA_p5 TGCAATTGCTCAGGATACACT TCTCGCGTGTCTACCATTTC 42.9 50.0 57.6 57.7 610 bp 1.0 67.0